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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
28/01/2015 |
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Data da última atualização: |
05/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOMES, G. M. F.; VASCONCELOS, A. M. de; EGITO, A. S. do; CARNEIRO, J. da C.; FONTELES, N. L. de O.; SALLES, H. O. |
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Afiliação: |
Gil Mario Ferreira Gomes; Ângela Maria de Vasconcelos; ANTONIO SILVIO DO EGITO, CNPC; Jailton da Costa Carneiro; Natália Lívia de Oliveira Fonteles; HEVILA OLIVEIRA SALLES, CNPC. |
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Título: |
Biodegradação do bagaço de cana-de-açúcar por microrganismos ruminais de caprinos e ovinos. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Bioscience Journal, Uberlândia, v. 31, n. 1, p. 204-214, jan./feb. 2015. |
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DOI: |
http://dx.doi.org/10.14393/BJ-v31n1a2015-22034 |
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Idioma: |
Português |
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Conteúdo: |
Resumo: No presente estudo objetivou-se avaliar a degradação do bagaço de cana-de-açúcar (BCA) integral (BIN) ou hidrolisado (BH) pela microbiota ruminal de caprinos e ovinos de raças naturalizadas do Nordeste brasileiro e o potencial desses animais como fontes de microrganismos e/ou enzimas celulolíticas para degradação da fibra do BCA. Para hidrólise do BCA foi utilizada uma solução de NaOH a 50%, 30% na matéria seca (MS). Foram determinadas as concentrações de MS, proteína bruta (PB), cinzas (CZ), fibra detergente neutro (FDN), fibra detergente ácido (FDA), celulose (Cel), hemicelulose (Hcel) e lignina (Lig). A degradação in situ da FDN foi determinada pela incubação ruminal em sacos de náilon do BH nos tempos: 0, 6, 24 e 96 horas. A técnica de duas etapas preconizada por Tilley e Terry foi utilizada para determinar a digestibilidade in vitro da MS (DIVMS). Foi coletado conteúdo ruminal dos animais quatro horas após a infusão de 100g de BIN via fístula ruminal para a separação de microrganismos associados às fases líquida e sólida, utilizando tampão fosfato de sódio 50 mM, pH 6,9. A fração sólida foi submetida ao cultivo in vitro com o substrato BIN, por 96 horas, para determinação da taxa de degradação da MS, FDN e atividade celulolítica. O pH foi determinado nos tempos de cultivo de 24, 48 e 96 horas. O pré-tratamento com NaOH aumentou a DIVMS (P<0,05). Não houve efeito de espécie sobre a DIVMS (P>0,05). A inclusão de NaOH aumentou a degradação in situ da FDN, apresentando os ovinos menor tempo de colonização (TC). Houve solubilização da Hcel, da Cel e da Lign no BCA pré-tratado com NaOH. A atividade celulolítica se concentrou na fração sólida independente da espécie doadora do inóculo, sendo observado crescimento microbiano no cultivo in vitro do BIN à partir dessa fração. O pH aumentou com o tempo de cultivo in vitro. Microrganismos ruminais de Caprinos e ovinos naturalizados do Nordeste brasileiro colonizaram e degradaram o BIN e BH. O NaOH pode ser utilizado no pré-tratamento alcalino do BCA. [Biodegradation of sugarcane bagasse by ruminal microorganisms from sheep and goats]. Abstract: The purpose of this study was to evaluate the biodegradation of sugar cane bagasse (SCB), whole (WB) or hydrolyzed (HB) by ruminal microorganisms from Brazilian naturalized goat and sheep breeds, and the animals? potential as a source of microorganisms and/or cellulolytic enzymes for the biodegradation of SCB fiber. SCB hydrolysis was performed using 50% sodium hydroxide solution on 30% of dry matter (DM). The concentrations of DM, crude protein (CP), ether extract (EE), ash, neutral detergent fiber (NDF), acid detergent fiber (ADF), cellulose (Cel), hemicellulose (Hcel) and lignin (Lig) were determined in the WB and HB. For the in situ biodegradation of NDF, the HB was placed in nylon bags and incubated in rumen for 0, 6, 24 and 96 hours. The two-step technique recommended by Tilley and Terry was used to determine the in vitro digestibility of DM (IVDDM). Four hours after infusion of WB (100 g) through the ruminal fistula, the rumen content was collected from the animals and the microorganisms associated with the liquid and solid phases were separated, the former by filtration and the latter by extraction with 50 mM sodium phosphate buffer, pH 6.9. The solid fraction was subjected to in vitro culture with WB substrate for 96 hours to determine the biodegradation rate of DM, NDF and cellulolytic activity. The pH was measured after culture times of 24, 48 and 96 hours. Pretreatment with NaOH increased the IVDDM (P <0.05). No species effect on the IVDDM was detected (P> 0.05). The addition of NaOH increased the in situ biodegradation rate of NDF, with sheep showing a lower lag time (LT). There was a solubilization of Hcel, Cel and Lig in SCB pretreated with NaOH. Cellulolytic activity was higher in the solid phase, irrespective of the inoculum source, and microbial growth was observed in in vitro cultures using microorganisms from the solid phase and WB as substrate. The pH of the in vitro culture increased over time. Ruminal microorganisms from Brazilian naturalized goat and sheep breeds successfully colonized and biodegraded both WB and HB. Sodium hydroxide proved useful as an alkaline pretreatment for SCB. MenosResumo: No presente estudo objetivou-se avaliar a degradação do bagaço de cana-de-açúcar (BCA) integral (BIN) ou hidrolisado (BH) pela microbiota ruminal de caprinos e ovinos de raças naturalizadas do Nordeste brasileiro e o potencial desses animais como fontes de microrganismos e/ou enzimas celulolíticas para degradação da fibra do BCA. Para hidrólise do BCA foi utilizada uma solução de NaOH a 50%, 30% na matéria seca (MS). Foram determinadas as concentrações de MS, proteína bruta (PB), cinzas (CZ), fibra detergente neutro (FDN), fibra detergente ácido (FDA), celulose (Cel), hemicelulose (Hcel) e lignina (Lig). A degradação in situ da FDN foi determinada pela incubação ruminal em sacos de náilon do BH nos tempos: 0, 6, 24 e 96 horas. A técnica de duas etapas preconizada por Tilley e Terry foi utilizada para determinar a digestibilidade in vitro da MS (DIVMS). Foi coletado conteúdo ruminal dos animais quatro horas após a infusão de 100g de BIN via fístula ruminal para a separação de microrganismos associados às fases líquida e sólida, utilizando tampão fosfato de sódio 50 mM, pH 6,9. A fração sólida foi submetida ao cultivo in vitro com o substrato BIN, por 96 horas, para determinação da taxa de degradação da MS, FDN e atividade celulolítica. O pH foi determinado nos tempos de cultivo de 24, 48 e 96 horas. O pré-tratamento com NaOH aumentou a DIVMS (P<0,05). Não houve efeito de espécie sobre a DIVMS (P>0,05). A inclusão de NaOH aumentou a degradação in situ da FDN, apresenta... Mostrar Tudo |
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Palavras-Chave: |
Cellulolytic enzymes; Degradação; Residues. |
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Thesagro: |
Bagaço; Cana de açúcar; Caprino; Digestibilidade In Vitro; Enzima celulolítica; Hidróxido de sódio; Ovino; Resíduo; Rúmen. |
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Thesaurus Nal: |
Bagasse; Goats; Sheep; Sodium hydroxide; Sugarcane. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/116724/1/cnpc-2014-Biodegradacao.pdf
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Marc: |
LEADER 05491naa a2200397 a 4500
001 2006969
005 2019-04-05
008 2015 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.14393/BJ-v31n1a2015-22034$2DOI
100 1 $aGOMES, G. M. F.
245 $aBiodegradação do bagaço de cana-de-açúcar por microrganismos ruminais de caprinos e ovinos.$h[electronic resource]
260 $c2015
520 $aResumo: No presente estudo objetivou-se avaliar a degradação do bagaço de cana-de-açúcar (BCA) integral (BIN) ou hidrolisado (BH) pela microbiota ruminal de caprinos e ovinos de raças naturalizadas do Nordeste brasileiro e o potencial desses animais como fontes de microrganismos e/ou enzimas celulolíticas para degradação da fibra do BCA. Para hidrólise do BCA foi utilizada uma solução de NaOH a 50%, 30% na matéria seca (MS). Foram determinadas as concentrações de MS, proteína bruta (PB), cinzas (CZ), fibra detergente neutro (FDN), fibra detergente ácido (FDA), celulose (Cel), hemicelulose (Hcel) e lignina (Lig). A degradação in situ da FDN foi determinada pela incubação ruminal em sacos de náilon do BH nos tempos: 0, 6, 24 e 96 horas. A técnica de duas etapas preconizada por Tilley e Terry foi utilizada para determinar a digestibilidade in vitro da MS (DIVMS). Foi coletado conteúdo ruminal dos animais quatro horas após a infusão de 100g de BIN via fístula ruminal para a separação de microrganismos associados às fases líquida e sólida, utilizando tampão fosfato de sódio 50 mM, pH 6,9. A fração sólida foi submetida ao cultivo in vitro com o substrato BIN, por 96 horas, para determinação da taxa de degradação da MS, FDN e atividade celulolítica. O pH foi determinado nos tempos de cultivo de 24, 48 e 96 horas. O pré-tratamento com NaOH aumentou a DIVMS (P<0,05). Não houve efeito de espécie sobre a DIVMS (P>0,05). A inclusão de NaOH aumentou a degradação in situ da FDN, apresentando os ovinos menor tempo de colonização (TC). Houve solubilização da Hcel, da Cel e da Lign no BCA pré-tratado com NaOH. A atividade celulolítica se concentrou na fração sólida independente da espécie doadora do inóculo, sendo observado crescimento microbiano no cultivo in vitro do BIN à partir dessa fração. O pH aumentou com o tempo de cultivo in vitro. Microrganismos ruminais de Caprinos e ovinos naturalizados do Nordeste brasileiro colonizaram e degradaram o BIN e BH. O NaOH pode ser utilizado no pré-tratamento alcalino do BCA. [Biodegradation of sugarcane bagasse by ruminal microorganisms from sheep and goats]. Abstract: The purpose of this study was to evaluate the biodegradation of sugar cane bagasse (SCB), whole (WB) or hydrolyzed (HB) by ruminal microorganisms from Brazilian naturalized goat and sheep breeds, and the animals? potential as a source of microorganisms and/or cellulolytic enzymes for the biodegradation of SCB fiber. SCB hydrolysis was performed using 50% sodium hydroxide solution on 30% of dry matter (DM). The concentrations of DM, crude protein (CP), ether extract (EE), ash, neutral detergent fiber (NDF), acid detergent fiber (ADF), cellulose (Cel), hemicellulose (Hcel) and lignin (Lig) were determined in the WB and HB. For the in situ biodegradation of NDF, the HB was placed in nylon bags and incubated in rumen for 0, 6, 24 and 96 hours. The two-step technique recommended by Tilley and Terry was used to determine the in vitro digestibility of DM (IVDDM). Four hours after infusion of WB (100 g) through the ruminal fistula, the rumen content was collected from the animals and the microorganisms associated with the liquid and solid phases were separated, the former by filtration and the latter by extraction with 50 mM sodium phosphate buffer, pH 6.9. The solid fraction was subjected to in vitro culture with WB substrate for 96 hours to determine the biodegradation rate of DM, NDF and cellulolytic activity. The pH was measured after culture times of 24, 48 and 96 hours. Pretreatment with NaOH increased the IVDDM (P <0.05). No species effect on the IVDDM was detected (P> 0.05). The addition of NaOH increased the in situ biodegradation rate of NDF, with sheep showing a lower lag time (LT). There was a solubilization of Hcel, Cel and Lig in SCB pretreated with NaOH. Cellulolytic activity was higher in the solid phase, irrespective of the inoculum source, and microbial growth was observed in in vitro cultures using microorganisms from the solid phase and WB as substrate. The pH of the in vitro culture increased over time. Ruminal microorganisms from Brazilian naturalized goat and sheep breeds successfully colonized and biodegraded both WB and HB. Sodium hydroxide proved useful as an alkaline pretreatment for SCB.
650 $aBagasse
650 $aGoats
650 $aSheep
650 $aSodium hydroxide
650 $aSugarcane
650 $aBagaço
650 $aCana de açúcar
650 $aCaprino
650 $aDigestibilidade In Vitro
650 $aEnzima celulolítica
650 $aHidróxido de sódio
650 $aOvino
650 $aResíduo
650 $aRúmen
653 $aCellulolytic enzymes
653 $aDegradação
653 $aResidues
700 1 $aVASCONCELOS, A. M. de
700 1 $aEGITO, A. S. do
700 1 $aCARNEIRO, J. da C.
700 1 $aFONTELES, N. L. de O.
700 1 $aSALLES, H. O.
773 $tBioscience Journal, Uberlândia$gv. 31, n. 1, p. 204-214, jan./feb. 2015.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
26/09/2022 |
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Data da última atualização: |
26/09/2022 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MACIEL, G. A.; FERNANDES, L. O.; REIS, N. J. C.; PAIVA, M. I. de; CORDEIRO, L. A. M. |
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Afiliação: |
GIOVANA ALCANTARA MACIEL, CPAC; LEONARDO OLIVEIRA FERNANDES; NÍNIVE JHORS CARNEIRO REIS; MARCIA INGRID DE PAIVA; LUIZ ADRIANO MAIA CORDEIRO, CPAC. |
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Título: |
BRS Paiaguás Grass Performance in different pastures systems in the Cerrado Region. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
In: REUNIÃO DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 57., 2022, Campinas. Tropical animal science and pratice to feed the planet: proceedings. Brasília, DF: SBZ; São Carlos, SP: Embrapa Pecuária Sudeste, 2022. |
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Páginas: |
p. 15 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of the experiment was to evaluate the effect of two pasture systems grazed by cattle on canopy height, forage mass and volumetric density of Urochloa brizantha BRS Paiaguás in the Cerrado Region. The experiment was conducted in the Experimental Farm of ABCZ during the execution of the Quality Meat Program, locate and 47º 57´ West Longitude, at an altitude of 774 m). The experimental period lasted from June of 2021 to March of 2022. In the warm months the mean temperature is 23.2º C, while in the cool months, the mean temperature is 19.4º C. The annual rainfall is 1685 mm and the mean humidity of the air is 71%. The grazing method was rotational stocking and forage allowance of 6 kg of dry mass 100 kg-1 of live weight day-1 during dry and wet season based on height and availability of dry grass mass. The treatments were: 1) SPS - Silvopastoral System with U. brizantha BRS Paiaguás integrated with eucalyptus trees (Eucalyptus citriodora and E. cloeziana) planted in 2013, in triple rows spaced 30 to 35 m and 1.5 m among trees, east-west direction, and pastures seeded in 2015/2016 crop season intercropped with maize for silage; and, 2) FS Full Sun pastures of U. brizantha BRS Paiaguás, intercropped with maize for silage in 2019/2020, with eighteen repetitions. Both treatments received topdressing nitrogen fertilization of 150 kg ha-1 split in three times during the wet season. To maintain planned forage allowance samplings of forage mass in the pre-grazing were made. The canopy height (cm) and forage mass were randomly sampled in 6 sites of 1 x 1 m at pre-grazing (kg DM ha-1). The volumetric density of forage (kg DM cm-1.ha-1) was estimated based in forage mass and canopy height. During experimental period 7 grazing cycles were conducted: 10/06/2021 22/08/2021, 23/08/2021-21/10/2021, 22/10/2021 11/11/2021, 12/11/2020 07/12/2021, 08/12/2021 11/01/2022, 12/01/2022 07/02/2022 and 08/02/2022 12/03/2022, respectively for cycles 1, 2, 3, 4, 5, 6 and 7. The experimental design was completely randomized with split plots repeated in time and the means were submitted to analysis of variance with Scott-Knott test at 5% probability. Canopy heighy was 8% higher in the SPS treatment (P<0.05), this behavior is due to the stretching in the grass caused by shading from the trees. Under these conditions, the plant grows in search of photosynthetically active radiation. However, there was a decrease of 15% in dry matter (P<0.05) in the SPS compared to grass established in FS treatment. The dry mass availability (kg ha-1 DM) was higher in the SPS (4.936 + 134.9) compared to that observed in FS (4.397 + 134.9). Thus the forage density expressed in kg cm- 1 ha-1 DM was not different among the systems evaluated. The loss of productivity of pasture intercropped with trees in integrated systems in relation to single pasture can be avoided according to the arrangement and amount of trees planted per hectare, which should allow the maximum input of solar radiation. MenosThe objective of the experiment was to evaluate the effect of two pasture systems grazed by cattle on canopy height, forage mass and volumetric density of Urochloa brizantha BRS Paiaguás in the Cerrado Region. The experiment was conducted in the Experimental Farm of ABCZ during the execution of the Quality Meat Program, locate and 47º 57´ West Longitude, at an altitude of 774 m). The experimental period lasted from June of 2021 to March of 2022. In the warm months the mean temperature is 23.2º C, while in the cool months, the mean temperature is 19.4º C. The annual rainfall is 1685 mm and the mean humidity of the air is 71%. The grazing method was rotational stocking and forage allowance of 6 kg of dry mass 100 kg-1 of live weight day-1 during dry and wet season based on height and availability of dry grass mass. The treatments were: 1) SPS - Silvopastoral System with U. brizantha BRS Paiaguás integrated with eucalyptus trees (Eucalyptus citriodora and E. cloeziana) planted in 2013, in triple rows spaced 30 to 35 m and 1.5 m among trees, east-west direction, and pastures seeded in 2015/2016 crop season intercropped with maize for silage; and, 2) FS Full Sun pastures of U. brizantha BRS Paiaguás, intercropped with maize for silage in 2019/2020, with eighteen repetitions. Both treatments received topdressing nitrogen fertilization of 150 kg ha-1 split in three times during the wet season. To maintain planned forage allowance samplings of forage mass in the pre-grazing were mad... Mostrar Tudo |
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Thesagro: |
Pastagem. |
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Thesaurus NAL: |
Silvopastoral systems. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1146805/1/Giovana-Maciel-BRS-Paiaguas.pdf
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Marc: |
LEADER 03748nam a2200193 a 4500
001 2146805
005 2022-09-26
008 2022 bl uuuu u00u1 u #d
100 1 $aMACIEL, G. A.
245 $aBRS Paiaguás Grass Performance in different pastures systems in the Cerrado Region.$h[electronic resource]
260 $aIn: REUNIÃO DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 57., 2022, Campinas. Tropical animal science and pratice to feed the planet: proceedings. Brasília, DF: SBZ; São Carlos, SP: Embrapa Pecuária Sudeste$c2022
300 $ap. 15
520 $aThe objective of the experiment was to evaluate the effect of two pasture systems grazed by cattle on canopy height, forage mass and volumetric density of Urochloa brizantha BRS Paiaguás in the Cerrado Region. The experiment was conducted in the Experimental Farm of ABCZ during the execution of the Quality Meat Program, locate and 47º 57´ West Longitude, at an altitude of 774 m). The experimental period lasted from June of 2021 to March of 2022. In the warm months the mean temperature is 23.2º C, while in the cool months, the mean temperature is 19.4º C. The annual rainfall is 1685 mm and the mean humidity of the air is 71%. The grazing method was rotational stocking and forage allowance of 6 kg of dry mass 100 kg-1 of live weight day-1 during dry and wet season based on height and availability of dry grass mass. The treatments were: 1) SPS - Silvopastoral System with U. brizantha BRS Paiaguás integrated with eucalyptus trees (Eucalyptus citriodora and E. cloeziana) planted in 2013, in triple rows spaced 30 to 35 m and 1.5 m among trees, east-west direction, and pastures seeded in 2015/2016 crop season intercropped with maize for silage; and, 2) FS Full Sun pastures of U. brizantha BRS Paiaguás, intercropped with maize for silage in 2019/2020, with eighteen repetitions. Both treatments received topdressing nitrogen fertilization of 150 kg ha-1 split in three times during the wet season. To maintain planned forage allowance samplings of forage mass in the pre-grazing were made. The canopy height (cm) and forage mass were randomly sampled in 6 sites of 1 x 1 m at pre-grazing (kg DM ha-1). The volumetric density of forage (kg DM cm-1.ha-1) was estimated based in forage mass and canopy height. During experimental period 7 grazing cycles were conducted: 10/06/2021 22/08/2021, 23/08/2021-21/10/2021, 22/10/2021 11/11/2021, 12/11/2020 07/12/2021, 08/12/2021 11/01/2022, 12/01/2022 07/02/2022 and 08/02/2022 12/03/2022, respectively for cycles 1, 2, 3, 4, 5, 6 and 7. The experimental design was completely randomized with split plots repeated in time and the means were submitted to analysis of variance with Scott-Knott test at 5% probability. Canopy heighy was 8% higher in the SPS treatment (P<0.05), this behavior is due to the stretching in the grass caused by shading from the trees. Under these conditions, the plant grows in search of photosynthetically active radiation. However, there was a decrease of 15% in dry matter (P<0.05) in the SPS compared to grass established in FS treatment. The dry mass availability (kg ha-1 DM) was higher in the SPS (4.936 + 134.9) compared to that observed in FS (4.397 + 134.9). Thus the forage density expressed in kg cm- 1 ha-1 DM was not different among the systems evaluated. The loss of productivity of pasture intercropped with trees in integrated systems in relation to single pasture can be avoided according to the arrangement and amount of trees planted per hectare, which should allow the maximum input of solar radiation.
650 $aSilvopastoral systems
650 $aPastagem
700 1 $aFERNANDES, L. O.
700 1 $aREIS, N. J. C.
700 1 $aPAIVA, M. I. de
700 1 $aCORDEIRO, L. A. M.
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